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Heartland Assays Inc. is a joint venture between Dr. Ronald Horst, Ames, IA and Dr. Bruce Hollis, Charleston, SC. HAI's main focus is to provide analytical services to research clientele and assist them in evaluating the vitamin D and calcium status of their research subjects. Recently there has been an explosion of interest in the role of vitamin D deficiency in the onset of many types of cancer and infectious diseases. Drs. Horst and Hollis have over 60 years combined experience in the development and application of vitamin D (and other calcium regulating hormone) assays in human and veterinary medicine.